Plasmid

Part:BBa_K4298012:Design

Designed by: Masixuan Zhao   Group: iGEM22_Peking   (2022-09-30)


pUC19-ITR-EPI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1000
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 505


Design Notes

pUC19 backbone, using Gibson assembly insert the EPI with α-secretion signal.


Source

Assembly with K4298009, K319003, K3105674, K4298005, K731721 and K4298011.